Penny Barton 1, Nick Robinson 1, Sonya Middleton 1, Amanda O’Brien 2, John Clarke 2, Maria Dominguez 1, Steve Gillgan 1, John Selmes 1 and Shelley Rhodes 1,*
1 Animal and Plant Health Agency (APHA), Weybridge KT15 3NB, UK; penny.barton@apha.gov.uk (P.B.); nicholas.robinson@apha.gov.uk (N.R.); sonya.middleton@apha.gov.uk (S.M.); maria.dominguez@apha.gov.uk (M.D.); stephen.gillgan@apha.gov.uk (S.G.); john.selmes@apha.gov.uk (J.S.)
2 Enfer Scientific, W91 FD74 Naas, County Kildare, Ireland; amandaobrien@enfergroup.com (A.O.);
johnclarke@enfergroup.com (J.C.)
* Correspondence: shelley.rhodes@apha.gov.uk
Simple Summary: This study describes the evaluation of four antibody tests (three ELISA tests and one lateral flow) for the detection of Mycobacterium bovis infection in deer and pigs. Test sensitivity and specificity were derived for each test with associated test cut-offs. There was a high level of test agreement between the tests. High test specificity was achieved, with a high to moderate test sensitivity, depending on whether or not a prior skin test had been performed, respectively. These data allow for the confident use of antibody tests for pigs and deer in GB, where previously none were available.